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EVEC Microgreens
Setup of an Extraction Method for the Analysis of Carotenoids in Microgreens
Microgreens are gaining increasing interest as a potential functional food due to their relevant contents of micronutrients and bioactive compounds, includ
Vito Michele Paradiso, Maria Castellino, Massimiliano Renna, Pietro Santamaria, Francesco Caponio
Foods · 2020 · https://doi.org/10.3390/foods9040459
Abstract
Microgreens are gaining increasing interest as a potential functional food due to their relevant contents of micronutrients and bioactive compounds, including carotenoids. Nevertheless, the analysis of carotenoids is inherently difficult, due to their thermal and chemical susceptibility, as well as to their varying polarity. From this point of view, extraction is the most critical step, compared to chromatographic separation and detection. Thus, the reliability of data on carotenoids should be guaranteed by a constant focus on analytical issues, with appropriate adaptations to each sample matrix. In this research, a specific extraction procedure for the analysis of carotenoids in microgreens was developed. Solvent composition, extraction time, solvent/sample ratio, and repeated extractions were evaluated. The obtained protocol showed recovery of 97.2%, limits of quantitation of 5.2 μg·g−1 for lutein and 15.9 μg·g−1 for β-carotene, as well as intra-day mean repeatability of 5.7% and inter-day mean repeatability of 4.7%.
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How to credit this work
Vito Michele Paradiso; Maria Castellino; Massimiliano Renna; Pietro Santamaria; Francesco Caponio (2020). Setup of an Extraction Method for the Analysis of Carotenoids in Microgreens. Foods. https://doi.org/10.3390/foods9040459 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.
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