Get in touch
Questions about growing, workshops, the cooperative or partnerships? Send us a message and we will reply by email.
Prefer email? Write to erasmus@evec.org.gr
EVEC Microgreens
Influence of contamination source and cultivation matrix on survival of Escherichia coli O157:H7 and Salmonella enterica on daikon microgreens
Consumption of microgreens has increased lately due to availability of bioactive compounds. However, they may present food safety risk similar to fresh pro
Chi‐Hung Chen, Hsin‐Bai Yin, Jitendra Patel
LWT · 2023 · https://doi.org/10.1016/j.lwt.2023.115165
Abstract
Consumption of microgreens has increased lately due to availability of bioactive compounds. However, they may present food safety risk similar to fresh produce and sprouts. Effect of cultivation matrixes and contamination routes on the survival of Escherichia coli O157:H7 (EHEC) and Salmonella enterica on daikon microgreen was investigated. Daikon microgreens contaminated with EHEC or Salmonella via seeds or irrigation water were cultivated on Composted cattle manure with soil (CG), Vermiculite perlite (VP), and Biostrate pads (BP) for 14 days. Surviving pathogens on microgreens and matrixes were determined by plating on selective agars. CG-grown microgreens (∼4.7 log CFU/g) showed significantly lower Salmonella populations compared to that recovered from BP-grown microgreens (∼6.5 log CFU/g) on day 14 when contaminated via seed. Similarly, recovery of Salmonella and EHEC from CG-grown microgreens was significantly lower than BP-grown microgreens on day 14 when contaminated via irrigation water. In addition, significantly higher populations of EHEC and Salmonella were recovered from BP (5.8 and 4.4 log CFU/g) matrix compared to these pathogens from CG (4.1 and 2.4 log CFU/g) on days 7 and 14, respectively. Results suggest that both contamination routes pose potential food safety risk as pathogens could survive on microgreens for 14 days.
Licence and reuse
| Licence | CC-BY-NC-ND-4.0 |
|---|---|
| May we host this PDF? | Yes |
| May we publish a plain-language summary? | No. This licence forbids derivative works. We may report and cite its findings, but not republish a rewritten version. |
| Commercial use permitted? | No — non-commercial use only |
| Share-alike required? | No |
How to credit this work
Chi‐Hung Chen; Hsin‐Bai Yin; Jitendra Patel (2023). Influence of contamination source and cultivation matrix on survival of Escherichia coli O157:H7 and Salmonella enterica on daikon microgreens. LWT. https://doi.org/10.1016/j.lwt.2023.115165 Licensed under Creative Commons Attribution NC ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.
Explore this on our site
- How to grow it: Chia microgreens — growing guide
- Related reading: International sanitary guidelines for the planting and distribution of microgreens and sprouts
About this copy
This is a copy of the published article, hosted by EVEC Athens for free educational use under its open licence. The permanent scientific reference is the DOI above. We have not altered the file in any way. If you are an author or rights holder and want this copy removed, please use our notice and takedown procedure. We acknowledge every request within five working days, and act within ten.
Co-funded by the European Union.
This portal presents the microgreens methodology; the organisation behind it is EVEC Athens — the European Voluntary and Educational Center (evec.org.gr). Views and opinions expressed are those of the author(s) only and do not necessarily reflect those of the European Union or the European Commission. Neither the European Union nor the granting authority can be held responsible for them.
Open licence. Except where stated otherwise, the educational materials on this portal are licensed under CC BY 4.0 — free to use, adapt and share, including commercially, with credit. See the licence terms.
Accessibility. We aim for WCAG 2.1 AA and publish what does and does not yet meet it — see the accessibility statement. If anything here blocks you, tell us and we will send the material in a format that works for you.