Get in touch
Questions about growing, workshops, the cooperative or partnerships? Send us a message and we will reply by email.
Prefer email? Write to erasmus@evec.org.gr
EVEC Microgreens
Substrate–Genotype Interaction Influences Growth and Phytochemical Composition of Wild and Commercial Purslane (Portulaca oleracea L.) Microgreens
Purslane is highly suitable for intensive microgreen cultivation due to its rapid growth, high germination rate, and exceptional nutritional profile, inclu
Ivana Kollárová, Ivana Mezeyová, Lucia Galovičová, Jana Žiarovská, Silvia Farkasová, Peter Pencák, Marcel Golian
Agronomy · 2025 · https://doi.org/10.3390/agronomy15092141
Abstract
Purslane is highly suitable for intensive microgreen cultivation due to its rapid growth, high germination rate, and exceptional nutritional profile, including omega-3 fatty acids, essential vitamins, and minerals. While previous studies have mostly emphasized its basic composition, our research investigated additional functional traits, such as pigment accumulation and antioxidant activity. We also explored the cultivation potential of a wild purslane genotype (G2), naturally growing in the Botanical Garden of the Slovak University of Agriculture in Nitra, as a sustainable alternative to commercially available seeds (G1). This study examined how genotype and substrate interactions influence growth performance, pigment concentration, and antioxidant capacity in Portulaca oleracea microgreens. Both genotypes were grown on two different substrates: agar mixed with perlite and mineral wool. Although conserved DNA-derived polymorphism marker analysis revealed a high degree of genetic similarity between G1 and G2, significant phenotypic differences were observed. G1 exhibited greater fresh biomass and shoot length, making it more visually appealing for commercial microgreen production. In contrast, G2 showed higher dry matter content and enhanced accumulation of chlorophylls and carotenoids. Antioxidant activity, measured by DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and FRAP (Ferric Reducing Antioxidant Power) assays, peaked in G1 cultivated on agar–perlite mix. These findings emphasize the importance of selecting the right genotype–substrate combination to optimize both quality and productivity in microgreen systems.
Licence and reuse
| Licence | CC-BY-4.0 |
|---|---|
| May we host this PDF? | Yes |
| May we publish a plain-language summary? | Yes — this licence permits adaptations. |
| Commercial use permitted? | Yes |
| Share-alike required? | No |
How to credit this work
Ivana Kollárová; Ivana Mezeyová; Lucia Galovičová; Jana Žiarovská; Silvia Farkasová; Peter Pencák; Marcel Golian (2025). Substrate–Genotype Interaction Influences Growth and Phytochemical Composition of Wild and Commercial Purslane (Portulaca oleracea L.) Microgreens. Agronomy. https://doi.org/10.3390/agronomy15092141 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.
Explore this on our site
- Related reading: Microgreens and their anti inflammatory antioxidant and preventive potential
- Related reading: Solanaceae lectins and alkaloids the compounds that make some microgreens toxic
- Related reading: The antioxidant capacity of radish microgreens
- Related reading: Microgreens the future of urban agriculture and superfoods part i
About this copy
This is a copy of the published article, hosted by EVEC Athens for free educational use under its open licence. The permanent scientific reference is the DOI above. We have not altered the file in any way. If you are an author or rights holder and want this copy removed, please use our notice and takedown procedure. We acknowledge every request within five working days, and act within ten.
Co-funded by the European Union.
This portal presents the microgreens methodology; the organisation behind it is EVEC Athens — the European Voluntary and Educational Center (evec.org.gr). Views and opinions expressed are those of the author(s) only and do not necessarily reflect those of the European Union or the European Commission. Neither the European Union nor the granting authority can be held responsible for them.
Open licence. Except where stated otherwise, the educational materials on this portal are licensed under CC BY 4.0 — free to use, adapt and share, including commercially, with credit. See the licence terms.
Accessibility. We aim for WCAG 2.1 AA and publish what does and does not yet meet it — see the accessibility statement. If anything here blocks you, tell us and we will send the material in a format that works for you.