Assess the impact of cultivation substrates for growing sprouts and microgreens of selected four legumes and two grains and evaluation of its nutritional properties

Many people suffer from a deficiency of essential micronutrients. Sprouts and microgreens can transform the whole idea of vegetables to resolve the need fo

Eswaranpillai Uma, Priyadharsini Murugesan, Ponmurugan Karuppiah

Plant Science Today · 2023 · https://doi.org/10.14719/pst.2058

Abstract

Many people suffer from a deficiency of essential micronutrients. Sprouts and microgreens can transform the whole idea of vegetables to resolve the need for a diet with fresh, nutrient-rich, and high content of phyto-compounds necessary for a healthy body. The study’s main objective is to evaluate the growth of 6 different seeds, such as four legumes; fenugreek, mung bean, cowpea, horse gram and two grains, wheat, sorghum microgreens. All the seeds were cultivated in soil, water and coco peat, to estimate and compare the nutritional properties of the selected sprouts vs. microgreens. The growth of microgreens in each medium was evaluated, and the proximate and nutritional properties were analysed. In terms of the growth of microgreens, coco peat medium serves the best, as it retains water for a long and it is porous to provide better aeration for the roots and also the day of harvest is shorter. In terms of the nutritional property of microgreens, soil serves the best, as it contains more nutrients than any other medium. The study results showed sprouts are better sources of proteins and carbohydrates than microgreens. However, microgreens were characterized by a high content of carotenoids, chlorophylls and ascorbic acid. It also exhibiting higher anti-diabetic and anticholinergic activity than sprouts. In addition, the microgreens have more micronutrients like zinc, copper, iron, magnesium, potassium etc., than the sprouts. Finally, microgreens were better growing with coco peat and also sources for functional components for dietary supplements and sustainable agriculture.

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Eswaranpillai Uma; Priyadharsini Murugesan; Ponmurugan Karuppiah (2023). Assess the impact of cultivation substrates for growing sprouts and microgreens of selected four legumes and two grains and evaluation of its nutritional properties. Plant Science Today. https://doi.org/10.14719/pst.2058 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Sanja Fabek Uher, Sanja Radman, Nevena Opačić, Mia Dujmović, Božidar Benko, Denis Lagundžija, Valent Mijić, Lucija Prša, Srđan Babac, Jana Šic Žlabur

Plants · 2023 · https://doi.org/10.3390/plants12112098

Abstract

Microgreens are young plants of various vegetables, medicinal and aromatic plants, cereals and edible wild plants that were first associated with nouvelle cuisine as decoration in dishes due to their attractive appearance and strong flavor. Recently, they have become more sought after in the market due to their high nutritional value. This is due to the growing interest of consumers in a healthy lifestyle that includes a varied diet with emphasis on fresh, functional foods. Nowadays, commercial production of microgreens is shifting to modern hydroponic systems due to their numerous advantages, such as accelerated plant growth and biomass production, earlier harvesting, and more production cycles that positively affect yield and chemical composition. Therefore, the aim of this study was to determine the content of specialized metabolites and antioxidant capacity of hydroponically grown alfalfa (Medicago sativa) cv. ‘Kangaroo’, yellow beet (Beta vulgaris var. conditiva) cv. ‘Yellow Lady’, red cabbage (Brassica oleracea L. var. rubra) cv. ‘Red Carpet’, and fennel (Foeniculum vulgare) cv. ‘Aganarpo’ microgreens. The highest content of total phenols (408.03 mg GAE/100 g fw), flavonoids (214.47 mg GAE/100 g fw), non-flavonoids (193.56 mg GAE/100 g fw) and ascorbic acid (74.94 mg/100 g fw) was found in fennel microgreens. The highest content of all analyzed chlorophyll pigments (Chl_a 0.536 mg/g fw, Chl_b 0.248 mg/g fw, and TCh 0.785 mg/g fw) was found in alfalfa microgreens. However, in addition to alfalfa, high levels of chlorophyll a (0.528 mg/g fw), total chlorophyll (0.713 mg/g fw) and the highest level of total carotenoids (0.196 mg/g fw) were also detected in fennel microgreens. The results suggest that microgreens grown on perlite in floating hydroponics have high nutritional potential as a functional food important for human health and therefore could be recommended for daily diet.

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Sanja Fabek Uher; Sanja Radman; Nevena Opačić; Mia Dujmović; Božidar Benko; Denis Lagundžija; Valent Mijić; Lucija Prša; Srđan Babac; Jana Šic Žlabur (2023). Alfalfa, Cabbage, Beet and Fennel Microgreens in Floating Hydroponics—Perspective Nutritious Food?. Plants. https://doi.org/10.3390/plants12112098 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Yumi Shirai, Yūkō Fujita, Ryota Hashimoto, Kazutaka Ohi, Hidenaga Yamamori, Yuka Yasuda, Tamaki Ishima, Hiroyuki Suganuma, Yusuke Ushida, Masatoshi Takeda, Kenji Hashimoto

PLoS ONE · 2015 · https://doi.org/10.1371/journal.pone.0127244

Abstract

Oxidative stress and inflammation play a role in cognitive impairment, which is a core symptom of schizophrenia. Furthermore, a hallmark of the pathophysiology of this disease is the dysfunction of cortical inhibitory γ-aminobutyric acid (GABA) neurons expressing parvalbumin (PV), which is also involved in cognitive impairment. Sulforaphane (SFN), an isothiocyanate derived from broccoli, is a potent activator of the transcription factor Nrf2, which plays a central role in the inducible expressions of many cytoprotective genes in response to oxidative stress. Keap1 is a cytoplasmic protein that is essential for the regulation of Nrf2 activity. Here, we found that pretreatment with SFN attenuated cognitive deficits, the increase in 8-oxo-dG-positive cells, and the decrease in PV-positive cells in the medial prefrontal cortex and hippocampus after repeated administration of phencyclidine (PCP). Furthermore, PCP-induced cognitive deficits were improved by the subsequent subchronic administration of SFN. Interestingly, the dietary intake of glucoraphanin (a glucosinolate precursor of SFN) during the juvenile and adolescence prevented the onset of PCP-induced cognitive deficits as well as the increase in 8-oxo-dG-positive cells and the decrease in PV-positive cells in the brain at adulthood. Moreover, the NRF2 gene and the KEAP1 gene had an epistatic effect on cognitive impairment (e.g., working memory and processing speed) in patients with schizophrenia. These findings suggest that SFN may have prophylactic and therapeutic effects on cognitive impairment in schizophrenia. Therefore, the dietary intake of SFN-rich broccoli sprouts during the juvenile and adolescence may prevent the onset of psychosis at adulthood.

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Yumi Shirai; Yūkō Fujita; Ryota Hashimoto; Kazutaka Ohi; Hidenaga Yamamori; Yuka Yasuda; Tamaki Ishima; Hiroyuki Suganuma; Yusuke Ushida; Masatoshi Takeda; Kenji Hashimoto (2015). Dietary Intake of Sulforaphane-Rich Broccoli Sprout Extracts during Juvenile and Adolescence Can Prevent Phencyclidine-Induced Cognitive Deficits at Adulthood. PLoS ONE. https://doi.org/10.1371/journal.pone.0127244 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Jafar K. Lone, Renu Pandey, C. Gayacharan

Heliyon · 2024 · https://doi.org/10.1016/j.heliyon.2024.e25870

Abstract

Escalating public health concerns necessitate innovative approaches to food sources. Microgreens, nutrient-rich seedlings of vegetables and herbs, have gained recognition as functional foods. This review explores the evolution of microgreens, cultivation methods, biochemical changes during germination, nutritional content, health benefits, and commercial significance. Comprehensive studies have demonstrated that microgreens have an elevated level of various nutrients. Further, in vitro and in vivo research validated their antioxidant, anticancer, antibacterial, anti-inflammatory, anti-obesity, and antidiabetic properties. Microgreens, termed “desert food,” show promise for sustainable food production in climate-vulnerable regions. This paper synthesizes recent research on microgreens, addressing challenges and gaps in understanding their nutritional content and health benefits. It contributes valuable insights for future research, fostering sustainable agriculture and enhancing understanding of microgreens in human health and nutrition.

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Jafar K. Lone; Renu Pandey; C. Gayacharan (2024). Microgreens on the rise: Expanding our horizons from farm to fork. Heliyon. https://doi.org/10.1016/j.heliyon.2024.e25870 Licensed under Creative Commons Attribution NC ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Bhornchai Harakotr, Lalita Charoensup, Panumart Rithichai, Yaowapha Jirakiattikul, Patlada Suthamwong

Resources · 2025 · https://doi.org/10.3390/resources14050071

Abstract

Selecting suitable crop species is crucial for optimizing the productivity and nutritional content of microgreens. In this study, twenty-three diverse microgreen species, grown under controlled conditions, were analyzed for yield, bioactive compounds, and antioxidant activities. The microgreens were cultivated on a peat substrate in a controlled environment, with a growth period of 6 to 20 days from planting to harvest. Conditions were maintained at 25 ± 2 °C, a 16 h photoperiod, CO2 concentration of 1000 ppm, relative humidity of 60 ± 2%, and the LED light was set at 330 μmol/m2/s PPFD. Results from the analysis revealed that the yield, bioactive compounds, and antioxidant potential differed significantly among the twenty-three microgreen species. Unfortunately, the superior microgreens exhibiting greater values for all studied traits could not be identified. However, the principal component analysis (PCA) clustered red radish, rat-tailed radish, and Chinese kale microgreens, which were high in both yield and bioactive compounds. In contrast, red holy basil and lemon basil microgreens had high levels of these compounds but low yields. Additionally, a high level of anti-tyrosinase activity was observed in garland chrysanthemum, Chinese mustard, and Chinese cabbage microgreens. Therefore, these microgreen species can be utilized individually or in varying ratios to produce bioactive compounds in different concentrations that are suitable for various applications. The information presented in this study provides valuable insights for health-conscious consumers and growers for selecting superior species with functional implications.

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Bhornchai Harakotr; Lalita Charoensup; Panumart Rithichai; Yaowapha Jirakiattikul; Patlada Suthamwong (2025). Yield, Bioactive Compounds, and Antioxidant Potential of Twenty-Three Diverse Microgreen Species Grown Under Controlled Conditions. Resources. https://doi.org/10.3390/resources14050071 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Isabelle Crowe, Karen Charlton, Anne‐Therese McMahon, Indiana Rhind, Katherine Kent

Journal of Human Nutrition and Dietetics · 2025 · https://doi.org/10.1111/jhn.70103

Abstract

AIMS: Nutrition and dietetics students are future change agents for sustainable food systems, yet sustainability education remains limited in university curricula. This study evaluated the impact of an experiential learning activity using an indoor aquaponic system on students’ knowledge, attitudes and behavioural intentions towards sustainable diets. METHODS: An embedded mixed methods pre-post survey captured baseline data on students’ sustainable diet knowledge, attitudes and behaviours, alongside diet quality (using the Australian Recommended Food Score). Students then participated in an interactive experiential learning activity involving an indoor aquaponic system. Post-activity surveys assessed changes in knowledge and behaviour intentions, as well as perceptions of the learning experience. Quantitative data were analysed using chi-square tests, regression and McNemar-Bowker tests; qualitative responses were thematically analysed. RESULTS: At baseline, students (n = 58, 87.9% female) reported limited knowledge of local food systems, though most valued sustainable food practices. Students who placed high importance on sustainable diets were significantly more likely to engage in sustainable dietary behaviours. Diet quality was significantly higher among students who grew their own food, bought locally or purchased seasonal produce (all p < 0.05). Participation in the experiential learning activity significantly improved students’ self-reported knowledge of local food systems (p < 0.001) and increased their intentions towards buying locally-grown (57.9% to 86.8%, p < 0.001) and growing food (36.8% to 78.9%, p < 0.001). Thematic analysis highlighted students’ increased awareness of sustainability complexity, their role as nutrition professionals and the importance of curriculum integration. CONCLUSION: Experiential learning with an indoor aquaponic system enhanced students’ knowledge and intentions related to sustainable diets. Students who engaged in sustainable behaviours had significantly higher diet quality, highlighting the potential dual benefit of curriculum integration. Embedding sustainability-focused learning in nutrition curricula may better prepare students to lead change in food systems.

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Isabelle Crowe; Karen Charlton; Anne‐Therese McMahon; Indiana Rhind; Katherine Kent (2025). The Impact of Experiential Learning Using an Indoor Aquaponic System on Nutrition Students’ Sustainable Food Knowledge and Behaviour. Journal of Human Nutrition and Dietetics. https://doi.org/10.1111/jhn.70103 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Onofrio Davide Palmitessa, Massimiliano Renna, Pasquale Crupi, Angelo Lovece, Filomena Corbo, Pietro Santamaria

Foods · 2020 · https://doi.org/10.3390/foods9050677

Abstract

Microgreens are gaining more and more interest, but little information is available on the effects of the chemical composition of the nutrient solution on the microgreen yield. In this study, three Brassica genotypes (B. oleracea var. italica, B. oleracea var. botrytis, and Brassica rapa L. subsp. sylvestris L. Janch. var. esculenta Hort) were fertigated with three modified strength Hoagland nutrient solutions (1/2, 1/4, and 1/8 strength) or with three modified half-strength Hoagland nutrient solutions with three different NH4:NO3 molar ratios (5:95, 15:85, and 25:75). Microgreen yields and content of inorganic ions, dietary fiber, proteins, α-tocopherol, and β-carotene were evaluated. Micro cauliflower showed the highest yield, as well as a higher content of mineral elements and α-tocopherol (10.4 mg 100 g−1 fresh weight (FW)) than other genotypes. The use of nutrient solution at half strength gave both a high yield (0.23 g cm−2) and a desirable seedling height. By changing the NH4:NO3 molar ratio in the nutrient solution, no differences were found on yield and growing parameters, although the highest β-carotene content (6.3 mg 100 g−1 FW) was found by using a NH4:NO3 molar ratio of 25:75. The lowest nitrate content (on average 6.8 g 100 g−1 dry weight) was found in micro broccoli and micro broccoli raab by using a nutrient solution with NH4:NO3 molar ratios of 25:75 and 5:95, respectively. Micro cauliflower fertigated with a NH4:NO3 molar ratio of 25:75 showed the highest dry matter (9.8 g 100 g−1 FW) and protein content (4.2 g 100 g−1 FW).

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Onofrio Davide Palmitessa; Massimiliano Renna; Pasquale Crupi; Angelo Lovece; Filomena Corbo; Pietro Santamaria (2020). Yield and Quality Characteristics of Brassica Microgreens as Affected by the NH4:NO3 Molar Ratio and Strength of the Nutrient Solution. Foods. https://doi.org/10.3390/foods9050677 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Leandro L. Coutinho, Tharcísio Citrangulo Tortelli, María Cristina Rangel

Frontiers in Oncology · 2023 · https://doi.org/10.3389/fonc.2023.1089115

Abstract

Cancer is a major public health concern worldwide responsible for high morbidity and mortality rates. Alternative therapies have been extensively investigated, and plant-derived compounds have caught the attention of the scientific community due to their chemopreventive and anticancer effects. Sulforaphane (SFN) is one of these naturally occurring agents, and studies have shown that it is able to target a specific cancer cell population displaying stem-like properties, known as cancer stem cells (CSCs). These cells can self-renewal and differentiate to form highly heterogeneous tumor masses. Notably, most of the conventional chemotherapeutic agents cannot target CSCs once they usually exist in a quiescent state and overall, the available cytotoxic drugs focus on highly dividing cells. This is, at least in part, one of the reasons why some oncologic patients relapse after standard therapy. In this review we bring together studies supporting not only the chemopreventive and anticancer properties of SFN, but especially the emerging anti-CSCs effects of this natural product and its potential to be used with conventional antineoplastic drugs in the clinical setting.

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Leandro L. Coutinho; Tharcísio Citrangulo Tortelli; María Cristina Rangel (2023). Sulforaphane: An emergent anti-cancer stem cell agent. Frontiers in Oncology. https://doi.org/10.3389/fonc.2023.1089115 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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