Microgreens of tropical edible-seed species, an economical source of phytonutrients: insights into nutrient content, growth environment and shelf life

Microgreens of six tropical edible-seed species categorized as pulse (green gram, horse gram), vegetable (mustard, amaranthus), millet (ragi) and cereal (w

K.S. Arya, M. Sangeeta Kutty, T. Pradeepkumar

Future Foods · 2023 · https://doi.org/10.1016/j.fufo.2023.100262

Abstract

Microgreens of six tropical edible-seed species categorized as pulse (green gram, horse gram), vegetable (mustard, amaranthus), millet (ragi) and cereal (wheat) were evaluated with an objective of studying the suitability of different growing media, growth condition, storage container and storage temperature. The six microgreens were also evaluated for the sensory qualities, nutrient composition, yield, seed to biomass ratio and benefit cost ratio. Yield and seedling height of microgreens were significantly influenced by the growing media, however there was no statistically significant difference in the nutrient composition of microgreens grown in the five different media. The fresh yield and dry yield of microgreens was recorded to be highest when grown on cocopeat media. Among the species, yield was highest for green gram microgreens, followed by mustard and horse gram. Rain shelter cultivation of microgreens promoted yield whereas the cultivation under room condition resulted in longer seedlings. The nutrient content (iron, calcium, beta carotene, protein, crude fibre, crude protein etc.) was higher in rain shelter grown microgreens. The microgreens grown indoor had better sensory qualities than when raised in rain shelter. Microgreens had higher shelf life when stored in PPE zip lock bags at lower temperature. Overall, microgreens of these tropical species can be an economic and nutritious supplement to regular vegetables.

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K.S. Arya; M. Sangeeta Kutty; T. Pradeepkumar (2023). Microgreens of tropical edible-seed species, an economical source of phytonutrients: insights into nutrient content, growth environment and shelf life. Future Foods. https://doi.org/10.1016/j.fufo.2023.100262 Licensed under Creative Commons Attribution NC ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Giandomenico Corrado, Christophe El‐Nakhel, Giulia Graziani, Antonio Pannico, Armando Zarrelli, P. Giannini, Alberto Ritieni, Stefania De Pascale, Marios C. Kyriacou, Youssef Rouphael

Horticulturae · 2021 · https://doi.org/10.3390/horticulturae7080211

Abstract

Neglected and underutilized species (NUS) offer largely unexplored opportunities for providing nutritious plant food, while making agro-ecosystems more diverse and resilient to climate change. The aim of this work was to explore the potential of two typical Mediterranean underutilized species, purslane and borage, as novel vegetable product (microgreens). Micro-scale production of edible plants is spreading due to the simplicity of their management, rapid cycle, harvest index, and phytochemical value of the edible product. Microgreens, therefore, represent an opportunity to link NUS, nutrition, and agricultural and dietary diversification. By analyzing yield, antioxidants activities, mineral composition, and main phenolic acids and flavonoids, our work indicated that the two species provide interesting results when compared with those reported for crops and horticultural species. Specifically, purslane should be considered highly nutritional due to the amount of phenolic compounds and ascorbic acid, and to potential good β-carotene bioavailability. Borage microgreens have a very high fresh yield and a more composite and balanced phenolic profile. In conclusion, our work provided evidence for implementing new ways to expand the NUS market-chains and for developing added-value food products.

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Giandomenico Corrado; Christophe El‐Nakhel; Giulia Graziani; Antonio Pannico; Armando Zarrelli; P. Giannini; Alberto Ritieni; Stefania De Pascale; Marios C. Kyriacou; Youssef Rouphael (2021). Productive and Morphometric Traits, Mineral Composition and Secondary Metabolome Components of Borage and Purslane as Underutilized Species for Microgreens Production. Horticulturae. https://doi.org/10.3390/horticulturae7080211 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Edi Nugroho, Aryanis Mutia Zahra, Rudiati Evi Masithoh, Hari K. Simatupang, A N K Sinaga, Novita D. Pitaloka, Muhammad Fahri Reza Pahlawan, L Rahmawati

IOP Conference Series Earth and Environmental Science · 2023 · https://doi.org/10.1088/1755-1315/1183/1/012049

Abstract

Abstract Microgreens are quickly-grown greens that are suitable for urban areas. The accumulation of chlorophyll in plants is essential for both nutritional compounds and the microgreen’s attractive appearance. A plant factory is a controlled-environment cultivation method that employs LEDs to enhance photosynthesis, vegetative development, seed germination, and chlorophyll accumulation. This work used VIS-NIR for the quantitative determination of chlorophyll content in green and red spinach microgreens grown under four different LED light intensities with four different ratios, with 100% blue, red, and white LED, and 67%:20%:13% of red:blue: white LED, as artificial grow for 12 hours illumination. The performance of MSC, AN, SNV, smoothing, and the first and second Savitzky-Golay’s derivatives (SGD) were examined. PLSR explained accurately predicted R 2 C, R 2 P, RMSEC, and RMSEP up to 0.957, 0.892, 1.054, and 1.661 for chlorophyll a using SGD 1st; 0.813, 0.652, 0.979, and 1.338 for chlorophyll b using SNV; and 0.931, 0.876, 1.155, and 1.550 for total chlorophyll using SNV. The wavelength region of 400 to 700 nm was dominant based on the best regression coefficient (β) for predicting green and red spinach microgreens. Results demonstrated that VIS-NIR spectroscopy, combined with chemometric techniques, PLSR, can be used to evaluate the chlorophyll content of green and red spinach microgreens.

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Edi Nugroho; Aryanis Mutia Zahra; Rudiati Evi Masithoh; Hari K. Simatupang; A N K Sinaga; Novita D. Pitaloka; Muhammad Fahri Reza Pahlawan; L Rahmawati (2023). Determination of green and red spinach microgreen chlorophyll content using visible spectroscopy and wavelength selection. IOP Conference Series Earth and Environmental Science. https://doi.org/10.1088/1755-1315/1183/1/012049 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Edi Nugroho, Aryanis Mutia Zahra, Rudiati Evi Masithoh, Hari K. Simatupang, A N K Sinaga, Novita D. Pitaloka, Muhammad Fahri Reza Pahlawan, L Rahmawati

IOP Conference Series Earth and Environmental Science · 2023 · https://doi.org/10.1088/1755-1315/1183/1/012049

Abstract

Abstract Microgreens are quickly-grown greens that are suitable for urban areas. The accumulation of chlorophyll in plants is essential for both nutritional compounds and the microgreen’s attractive appearance. A plant factory is a controlled-environment cultivation method that employs LEDs to enhance photosynthesis, vegetative development, seed germination, and chlorophyll accumulation. This work used VIS-NIR for the quantitative determination of chlorophyll content in green and red spinach microgreens grown under four different LED light intensities with four different ratios, with 100% blue, red, and white LED, and 67%:20%:13% of red:blue: white LED, as artificial grow for 12 hours illumination. The performance of MSC, AN, SNV, smoothing, and the first and second Savitzky-Golay’s derivatives (SGD) were examined. PLSR explained accurately predicted R 2 C, R 2 P, RMSEC, and RMSEP up to 0.957, 0.892, 1.054, and 1.661 for chlorophyll a using SGD 1st; 0.813, 0.652, 0.979, and 1.338 for chlorophyll b using SNV; and 0.931, 0.876, 1.155, and 1.550 for total chlorophyll using SNV. The wavelength region of 400 to 700 nm was dominant based on the best regression coefficient (β) for predicting green and red spinach microgreens. Results demonstrated that VIS-NIR spectroscopy, combined with chemometric techniques, PLSR, can be used to evaluate the chlorophyll content of green and red spinach microgreens.

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Edi Nugroho; Aryanis Mutia Zahra; Rudiati Evi Masithoh; Hari K. Simatupang; A N K Sinaga; Novita D. Pitaloka; Muhammad Fahri Reza Pahlawan; L Rahmawati (2023). Determination of green and red spinach microgreen chlorophyll content using visible spectroscopy and wavelength selection. IOP Conference Series Earth and Environmental Science. https://doi.org/10.1088/1755-1315/1183/1/012049 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Atsushi Saito, S Ishikawa, Kun Yang, Akira Sawa, Koko Ishizuka

Journal of Nutritional Science · 2025 · https://doi.org/10.1017/jns.2025.10033

Abstract

Sulforaphane (SFN), a bioactive compound derived from glucoraphanin in cruciferous vegetables such as broccoli, has been extensively studied for its therapeutic potential across diverse disease categories. SFN exerts its effects through well-characterised pathways, including the Keap1/Nrf2 axis, which regulates phase II detoxification enzymes, and epigenetic mechanisms such as histone deacetylase inhibition. This review evaluates clinical trials registered on ClinicalTrials.gov, focusing on those using SFN or broccoli-derived extracts. As a result, we identified 84 trials, of which 39 have been published. Results suggest SFN’s potential in regulating redox and inflammatory pathways, improving metabolic and cardiovascular outcomes, and exerting anti-cancer and neuroprotective effects. For healthy subjects, SFN enhanced detoxification and reduced inflammation. In cancer patients, SFN showed promise in early-stage prostate and breast cancer, particularly in GSTM1-positive individuals, but had limited effects in advanced cases. For brain disorders, SFN demonstrated symptomatic improvements in autism spectrum disorder and cognitive benefits in schizophrenia but lacked robust biomarker integration. SFN had minimal impact on respiratory diseases but showed supportive roles in allergic rhinitis therapy. Metabolic disease studies revealed glycaemic control improvements in type 2 diabetes but no benefits for hypertension. Approximately 50% of completed trials remain unpublished, raising concerns about publication bias. While published results highlight SFN’s therapeutic potential, limited sample sizes and inconsistent outcomes underscore the need for more extensive, stratified trials. This review emphasises the importance of integrating mechanistic insights and precision medicine approaches to maximise SFN’s clinical utility.

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Atsushi Saito; S Ishikawa; Kun Yang; Akira Sawa; Koko Ishizuka (2025). Sulforaphane as a Potential Therapeutic Agent: A Comprehensive Analysis of Clinical Trials and Mechanistic Insights. Journal of Nutritional Science. https://doi.org/10.1017/jns.2025.10033 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Oday Alrifai, Lili Mats, Ronghua Liu, Xiuming Hao, Massimo F. Marcone, Rong Tsao

Food Production Processing and Nutrition · 2021 · https://doi.org/10.1186/s43014-021-00072-y

Abstract

Abstract As of recent, microgreen vegetable production in controlled environments are being investigated for their bioactive properties. Phytochemicals like glucosinolates (GLS) are highly sensitive to varying spectral qualities of light, especially in leafy greens of Brassica where the responses are highly species-dependent. The accumulation of bioactive GLS were studied under 8 different treatments of combined amber (590 nm), blue (455 nm), and red (655 nm) light-emitting diodes (rbaLED). A semi-targeted metabolomics approach was carried out to profile common intact-GLS in microgreen extracts of Brassica by means of LC-HRMS/MS. Thirteen GLS were identified, among them were 8 aliphatic, 4 indolic and 1 aromatic GLS. Mass spectrometry data showed sinigrin had the highest average concentration and was highest in B. juncea , progoitrin was highest in B. rapa and glucobrassicin in R. sativus . The individual and total GLS in the microgreens of the present study were largely different under rbaLED; B. rapa microgreens contained the highest profile of total GLS, followed by R. sativus and B. juncea . Sinigrin was increased and gluconasturtiin was decreased under rbaLED lighting in most microgreens, glucoalyssin uniquely increased in R. sativus and decreased in B. rapa and glucobrassicin uniquely decreased in both B. rapa and B. juncea . The present study showed that rbaLED contributed to the altered profiles of GLS resulting in their significant modulation. Optimizing the light spectrum for improved GLS biosynthesis could lead to production of microgreens with targeted health-promoting properties. Graphical Abstract

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Oday Alrifai; Lili Mats; Ronghua Liu; Xiuming Hao; Massimo F. Marcone; Rong Tsao (2021). Effect of combined light-emitting diodes on the accumulation of glucosinolates in Brassica microgreens. Food Production Processing and Nutrition. https://doi.org/10.1186/s43014-021-00072-y Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Marco Garegnani, Carla Sandri, Claudia Pacelli, Francesca Ferranti, Elisabetta Bennici, Angiola Desiderio, Luca Nardi, Maria Elena Villani

Frontiers in Plant Science · 2024 · https://doi.org/10.3389/fpls.2023.1289208

Abstract

Introduction: The future of human space missions relies on the ability to provide adequate food resources for astronauts and also to reduce stress due to the environment (microgravity and cosmic radiation). In this context, microgreens have been proposed for the astronaut diet because of their fast-growing time and their high levels of bioactive compounds and nutrients (vitamins, antioxidants, minerals, etc.), which are even higher than mature plants, and are usually consumed as ready-to-eat vegetables. Methods: Our study aimed to identify the best light recipe for the soilless cultivation of two cultivars of radish microgreens (Raphanus sativus, green daikon, and rioja improved) harvested eight days after sowing that could be used for space farming. The effects on plant metabolism of three different light emitting diodes (LED) light recipes (L1-20% red, 20% green, 60% blue; L2-40% red, 20% green, 40% blue; L3-60% red, 20% green, 20% blue) were tested on radish microgreens hydroponically grown. A fluorimetric-based technique was used for a real-time non-destructive screening to characterize plant methabolism. The adopted sensors allowed us to quantitatively estimate the fluorescence of flavonols, anthocyanins, and chlorophyll via specific indices verified by standardized spectrophotometric methods. To assess plant growth, morphometric parameters (fresh and dry weight, cotyledon area and weight, hypocotyl length) were analyzed. Results: We observed a statistically significant positive effect on biomass accumulation and productivity for both cultivars grown under the same light recipe (40% blue, 20% green, 40% red). We further investigated how the addition of UV and/or far-red LED lights could have a positive effect on plant metabolite accumulation (anthocyanins and flavonols). Discussion: These results can help design plant-based bioregenerative life-support systems for long-duration human space exploration, by integrating fluorescence-based non-destructive techniques to monitor the accumulation of metabolites with nutraceutical properties in soilless cultivated microgreens.

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Marco Garegnani; Carla Sandri; Claudia Pacelli; Francesca Ferranti; Elisabetta Bennici; Angiola Desiderio; Luca Nardi; Maria Elena Villani (2024). Non-destructive real-time analysis of plant metabolite accumulation in radish microgreens under different LED light recipes. Frontiers in Plant Science. https://doi.org/10.3389/fpls.2023.1289208 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Ilaria Marchioni, Marco Martinelli, Roberta Ascrizzi, Costanza Gabbrielli, Guido Flamini, Luisa Pistelli, Laura Pistelli

Foods · 2021 · https://doi.org/10.3390/foods10020427

Abstract

Microgreens are the seedlings of herbs and vegetables which are harvested at the development stage of their two cotyledonary leaves, or sometimes at the emergence of their rudimentary first pair of true leaves. They are functional foods, the consumption of which is steadily increasing due to their high nutritional value. The species of the Brassicaceae family are good sources of bioactive compounds, with a favorable nutritional profile. The present study analyzed some phytochemical compounds with nutritional values, such as chlorophylls, polyphenols, carotenoids, anthocyanins, ascorbic acid, total and reducing sugars, and the antioxidant activity of five Brassicaceae species: broccoli (Brassica oleracea L.), daikon (Raphanus raphanistrum subsp. sativus (L.) Domin), mustard (Brassica juncea (L.) Czern.), rocket salad (Eruca vesicaria (L.) Cav.), and watercress (Nasturtium officinale R.Br.). Broccoli had the highest polyphenol, carotenoid and chlorophyll contents, as well as a good antioxidant ability. Mustard was characterized by high ascorbic acid and total sugar contents. By contrast, rocket salad exhibited the lowest antioxidant content and activity. The essential oil (EO) composition of all of these species was determined in order to identify their profile and isothiocyanates content, which are compounds with many reported health benefits. Isothiocyanates were the most abundant group in broccoli (4-pentenyl isothiocyanate), mustard (allyl isothiocyanate), and watercress (benzyl isothiocyanate) EOs, while rocket salad and daikon exhibited higher contents of monoterpene hydrocarbons (myrcene) and oxygenated diterpenes (phytol), respectively. Broccoli microgreens exhibited the overall best nutritional profile, appearing as the most promising species to be consumed as a functional food among those analyzed.

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Ilaria Marchioni; Marco Martinelli; Roberta Ascrizzi; Costanza Gabbrielli; Guido Flamini; Luisa Pistelli; Laura Pistelli (2021). Small Functional Foods: Comparative Phytochemical and Nutritional Analyses of Five Microgreens of the Brassicaceae Family. Foods. https://doi.org/10.3390/foods10020427 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Marios C. Kyriacou, Christophe El‐Nakhel, Antonio Pannico, Giulia Graziani, Georgios A. Soteriou, Maria Giordano, Armando Zarrelli, Alberto Ritieni, Stefania De Pascale, Youssef Rouphael

Frontiers in Plant Science · 2019 · https://doi.org/10.3389/fpls.2019.01501

Abstract

Advanced analytical data on microgreens’ response to different light spectra constitutes a valuable resource for designing future crop-specific spectral management systems. The current study defined variation in productivity, nutritive and functional quality (mineral-carotenoid-polyphenolic profiles and antioxidant capacity) of novel microgreens (amaranth, cress, mizuna, purslane) in response to select spectral bandwidths (red, blue, blue-red) and appraised clustering patterns configured by the genotype-light-spectrum nexus. Growth parameters dependent on primary metabolism were most favoured by blue-red light’s efficiency in activating the photosynthetic apparatus. Nitrate accumulation was higher under monochromatic light owing to the dependency of nitrite reductase on the light-driven activity of PSI, most efficiently promoted by blue-red light. Although mineral composition was mostly genotype-dependent, monochromatic red and blue lights tended to increase K and Na and decrease Ca and Mg concentrations. Lutein, β-carotene and lipophilic antioxidant capacity were generally increased by blue-red light putatively due to the coupling of heightened photosynthetic activity to increased demand for protection against oxidative stress; the disparate response however of purslane highlights the importance of genotype specificity in these responses and calls for additional investigation. Analysis of polyphenols by Orbitrap LC-MS/MS revealed substantial genotypic differences. Most abundant phenolics were chlorogenic acid (x̄= 5503 µg g-1 dw), feruloylquinic acid (x̄= 974.1 µg g-1 dw) and caffeoylferuloyl tartaric acid (x̄= 5503 µg g-1 dw). Hydroxycinnamic acids accounted for 79.0% of the mean total phenolic content across species, flavonol glycosides for 20.7% and flavone glycosides for 0.3%. The general response across species was a decrease in individual polyphenolic constituents, particularly flavonol glycosides, and total polyphenols under blue-red light. The pronounced effectiveness of monochromatic blue light in eliciting synthesis of flavonoids could be linked to their capacity for absorbing shorter wavelengths thereby quenching generated photo-oxidation potential. The light-induced stimulation of the phenylpropanoid pathway by monochromatic blue light through epigenetic mechanisms or redox signalling in the photosynthetic apparatus warrants further investigation. The current work highlights how optimized genetic background combined with effective light management might facilitate the production of superior functional quality microgreens.

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Marios C. Kyriacou; Christophe El‐Nakhel; Antonio Pannico; Giulia Graziani; Georgios A. Soteriou; Maria Giordano; Armando Zarrelli; Alberto Ritieni; Stefania De Pascale; Youssef Rouphael (2019). Genotype-Specific Modulatory Effects of Select Spectral Bandwidths on the Nutritive and Phytochemical Composition of Microgreens. Frontiers in Plant Science. https://doi.org/10.3389/fpls.2019.01501 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Ilaria Marotti, Francesca Truzzi, Camilla Tibaldi, Lorenzo Negri, Giovanni Dinelli

AIMS Agriculture and Food · 2020 · https://doi.org/10.3934/agrfood.2021001

Abstract

Literature on microgreens, an emerging new functional food crop, remains limited. Further study on microgreens as a promising dietary component for potential use in diet-based disease prevention is, therefore, essential. Given that the anti-inflammatory and anti-oxidant properties of mature licorice root material are well-documented, the objective of the present, preliminary study was to present licorice (<em>Glycyrrhiza glabra</em> L.) as a novel, edible fresh-food microgreen candidate. The effect of leaf, stem and root polyphenol extracts of 20-day old licorice microgreen seedlings on cell proliferation and viability of Caco-2 cells (simulating the intestinal epithelium), after pro-inflammatory induction of lipopolysaccharide (LPS), was examined and then compared to the polyphenol, flavonoid and anti-radical activities of the respective tissue extracts. Root extracts contained a two-fold less polyphenol (including flavonoid) content compared to leaf extracts, but with a five-fold lower anti-radical scavenging activity. Only the root extracts provided functional protection in terms of preserving cell proliferation and viability of LPS-treated Caco-2 cells. Some protection was also afforded by the stems, but the young leaf material offered no anti-inflammatory protection. Results point to a differing composition of anti-inflammatory polyphenols between the root and leaf, suggesting that the protective efficacy of the root extracts (and to some degree, the stem extracts) resides in inhibiting the pro-inflammatory cascade and resultant cytotoxic effects as opposed to a direct anti-radical scavenging activity. Potential use of licorice as a microgreen is promising, but will necessitate further study.

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Ilaria Marotti; Francesca Truzzi; Camilla Tibaldi; Lorenzo Negri; Giovanni Dinelli (2020). Evaluation of licorice (Glycyrrhiza glabra L.) as a novel microgreen from the anti-inflammatory potential of polyphenols. AIMS Agriculture and Food. https://doi.org/10.3934/agrfood.2021001 Licensed under Creative Commons Attribution NC SA 4.0 (https://creativecommons.org/licenses/by-nc-sa/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Sylvia Lee, Kiri Michell, Michelle Butler, Brayden T. Smith, Emily K. Woolf, S. Holmes, Lauren E. Grabos, Allegra R. Vazquez, Hanan Isweiri, Marisa Bunning, Mark Uchanski, Sangeeta Rao, Steven E. Newman, Tiffany L. Weir, Sarah A. Johnson

Nutrients · 2025 · https://doi.org/10.3390/nu17030467

Abstract

Background/Objectives: Microgreens are rich in nutrients and phytochemicals that can support healthy aging, including attenuation of cardiovascular disease risk. The nutrient and phytochemical contents of red beet (i.e., bull’s blood’ beet, Beta vulgaris) and red cabbage (Brassica oleracea var capitate) microgreens, as well as existing preclinical evidence suggest their cardioprotective effects, but the feasibility, gastrointestinal tolerability, and human health effects of daily microgreen consumption are unknown. This study aimed to evaluate the feasibility and gastrointestinal tolerability of 2 weeks of daily microgreen consumption in healthy middle-aged/older (MA/O) adults. A secondary aim was to characterize potential health effects. Methods: Healthy MA/O adults (initial n = 26) were randomized to consume either 2 cups of ‘bull’s blood’ beet or red cabbage microgreens daily for 2 weeks in a crossover design, with each treatment period separated by 2 weeks. Feasibility was determined through participant retention and intervention compliance (i.e., total doses consumed divided by 14 days), while gastrointestinal tolerability was determined by a gastrointestinal health questionnaire and bowel movement log. Impacts of microgreen consumption on brachial and aortic hemodynamic parameters, and gut microbiota composition were evaluated. Results: Daily consumption for 2 weeks of ‘bull’s blood’ beet and red cabbage microgreens was found to be feasible as indicated by high participant retention (final n = 24) and overall treatment compliance of 95.6%. Gastrointestinal symptom severity was not impacted overall, though an improvement in gastrointestinal inflammation-associated symptom severity scores following the red cabbage microgreen intervention (p = 0.047) was observed. There were no changes in bowel movement quality, hemodynamic parameters, or on alpha or beta diversity of the gut microbiota. Conclusions: Daily consumption of ‘bull’s blood’ beet and red cabbage microgreens is feasible and tolerable in healthy MA/O adults. Future studies designed to evaluate their health impacts are needed.

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Sylvia Lee; Kiri Michell; Michelle Butler; Brayden T. Smith; Emily K. Woolf; S. Holmes; Lauren E. Grabos; Allegra R. Vazquez; Hanan Isweiri; Marisa Bunning; Mark Uchanski; Sangeeta Rao; Steven E. Newman; Tiffany L. Weir; Sarah A. Johnson (2025). Feasibility and Tolerability of Daily Microgreen Consumption in Healthy Middle-Aged/Older Adults: A Randomized, Open-Label, Controlled Crossover Trial. Nutrients. https://doi.org/10.3390/nu17030467 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Irma Ortiz, Xudong Zhu, Shirin Shakoomahally, Wenyi Wu, Olayanju Kunle-Rabiu, Ellen R. Turner, Tianbao Yang

Technology in Horticulture · 2024 · https://doi.org/10.48130/tihort-0023-0031

Abstract

Microgreens are 10-20-day old young vegetables with rich nutrition. In commercial settings, their harvest time varies after first true leaf emerges. To determine the optimal harvest time, the microgreen yield and quality of broccoli (<italic>Brassica oleracea</italic> var. <italic>italica</italic> Plenck) and radish (<italic>Rhaphanus sativus</italic> L.), were analyzed after different harvest times. Under hydroponic culture at 25 °C and 16/8h photoperiod, the first true leaf of broccoli and radish emerged on day 10 and day 7 respectively. Broccoli harvested at day 13 and radish harvested at day 10, showed significantly highest yield and chlorophyll content compared to that of plants harvested at any other day. The microgreen yield was 20% higher when broccoli and radish were harvested at day 13 and day 10 compared to those harvested on earlier days. The overall visual quality of microgreens stored at 4 °C was evaluated for the extent of decay. There was less visible decay for broccoli harvested at day 13 and radish harvested at day 10. Regarding nutrient quality, there was no significant difference for glucosinolates and total phenolics among different harvested days. However, anthocyanins were decreased significantly for radish harvested at day 11. Overall, the results suggest that the best harvest time for radish and broccoli microgreens is when about 75% true leaves emerge. This research will provide microgreen growers a basis for determining the optimal harvest time of microgreens.

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Irma Ortiz; Xudong Zhu; Shirin Shakoomahally; Wenyi Wu; Olayanju Kunle-Rabiu; Ellen R. Turner; Tianbao Yang (2024). Effects of harvest day after first true leaf emergence of broccoli and radish microgreen yield and quality. Technology in Horticulture. https://doi.org/10.48130/tihort-0023-0031 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Yuan Zhong, Zhilong Jia, Hailong Zhou, Dan Zhang, Guichen Li, Jihua Yu

International Journal of Molecular Sciences · 2023 · https://doi.org/10.3390/ijms241914988

Abstract

The ultrasonic cell disruption method was used to efficiently extract isothiocyanates and other volatile compounds from radish microgreens. A total of 51 volatiles were identified and quantified by headspace solid-phase micro-extraction and gas chromatography-mass spectrometry (HS-SPME/GC-MS) in four radish microgreen cultivars, mainly including alcohols, aldehydes, isothiocyanates, sulfides, ketones, esters, terpenes, and hydrocarbons. The correlation between cultivars and volatile compounds was determined by chemometrics analysis, including principal component analysis (PCA) and hierarchical clustering heat maps. The aroma profiles were distinguished based on the odor activity value (OAV), odor contribution rate (OCR), and radar fingerprint chart (RFC) of volatile compounds. This study not only revealed the different flavor characteristics in four cultivars but also established a theoretical basis for the genetic improvement of radish microgreen flavors.

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Yuan Zhong; Zhilong Jia; Hailong Zhou; Dan Zhang; Guichen Li; Jihua Yu (2023). Comparative Analysis of Volatile Compounds from Four Radish Microgreen Cultivars Based on Ultrasonic Cell Disruption and HS-SPME/GC–MS. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms241914988 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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L. Kowitcharoen, Surisa Phornvillay, Pornpan Lekkham, Nutthachai Pongprasert, V. Srilaong

Applied Sciences · 2021 · https://doi.org/10.3390/app11177981

Abstract

Microgreens are young and tender leafy vegetables that have gained wider consumer acceptance. This is attributed to their low caloric composition and rich micronutrient and antioxidant composition. The present study investigated the bioactive composition and proximate analysis of fourteen microgreens belonging to Brassicaceae, Fabaceae, Pedaliaceae, Polygonaceae, Convolvulaceae, and Malvaceae. All the microgreens showed low calories (20.22 to 53.43 kcal 100 g−1) and fat (0.15 to 0.66 g 100 g−1), whilst mung bean and lentil microgreens showed considerable amounts of carbohydrate (7.16 g 100 g−1) and protein (6.47 g 100 g−1), respectively. Lentil microgreens had the highest total chlorophyll (112.62 mg 100 g−1) and carotenoid (28.37 mg 100 g−1) contents, whilst buckwheat microgreens showed the highest total phenolic content (268.99 mg GAE 100 g−1) and DPPH• scavenging activity (90.83 mM TEAC g−1). The lentil microgreens also presented high ascorbic acid content (128.70 mg 100 g−1) along with broccoli, Chinese kale, purple radish, and red cabbage microgreens (79.11, 81.33, 82.58, and 89.49 mg 100 g−1, respectively). Anthocyanin content was only detected in purple radish (0.148 mg CGE 100 g−1) and red cabbage (0.246 mg CGE 100 g−1). The results provide basic information and highlight the benefits of utilizing genetic biodiversity to obtain microgreens with the desired nutrients and antioxidants.

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L. Kowitcharoen; Surisa Phornvillay; Pornpan Lekkham; Nutthachai Pongprasert; V. Srilaong (2021). Bioactive Composition and Nutritional Profile of Microgreens Cultivated in Thailand. Applied Sciences. https://doi.org/10.3390/app11177981 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Manyasha L. L. Ntsoane, Vimbainashe E. Manhivi, Tinotenda Shoko, Faith Seke, Yasmina Sultanbawa, Dharini Sivakumar

International Journal of Food Science & Technology · 2024 · https://doi.org/10.1111/ijfs.16890

Abstract

Summary Microgreens grow best under light‐emitting diodes (LED), an artificial light source. In this study, LED lights (red, blue and far‐red) were tested on brassica microgreens to see if they induced an increase in bioactive compounds (glucosinolates and phenolics). In vitro digestion also measured bioavailable bioactive compounds in the intestinal phase. LED lights (red, blue and far‐red) were applied for 6 h in storage at 5 °C and 85% RH for 5 days on cabbage ( Brassica oleracea ), radish ( Raphanus sativus ) and rocket ( Eruca vesicaria (L.) Cav). Red light significantly enhanced ascorbic acid, total phenols, kaempferol and quercetin glycoside concentrations in all three Brassica microgreens and antioxidant activities. Exposure to red LED light increased 4‐methoxyglucobrassicin (cabbage), glucoraphenin (radish), glucoraphanin A and glucoerucin (rocket). Red light stress may have resulted in secondary metabolite production in immature plants. An in vitro digestion showed higher concentrations of phenolic compounds, glucosinolate components and antioxidants in the intestinal phase. Red LED light is recommended as a postharvest treatment for improving cabbage, rocket and radish bioactive compounds.

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Manyasha L. L. Ntsoane; Vimbainashe E. Manhivi; Tinotenda Shoko; Faith Seke; Yasmina Sultanbawa; Dharini Sivakumar (2024). Brassica microgreens cabbage (Brassica oleracea), radish (Raphanus sativus) and rocket (Eruca vesicaria) (L.) Cav: application of red-light emitting diodes lighting during postharvest storage and in vitro digestion on bioactive compounds and antioxid. International Journal of Food Science & Technology. https://doi.org/10.1111/ijfs.16890 Licensed under Creative Commons Attribution NC ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Jagarlamudi Nethra, B. Srinivasulu, Vadada Vinay Kumar, C. Lakshmana Rao

International Journal of Environment and Climate Change · 2024 · https://doi.org/10.9734/ijecc/2024/v14i124615

Abstract

In this comprehensive review, we delve into the multifaceted world of nutrient-rich microgreens, with a particular emphasis on their significance in urban agriculture. By exploring their nutritional composition, culinary applications and cultivation techniques, we aim to shed light on the transformative potential of microgreens in promoting sustainable urban food systems. Through an interdisciplinary lens encompassing agronomy, nutrition and urban planning, we uncover the myriad benefits of microgreens cultivation and highlight the critical role they play in shaping the future of urban agriculture.

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Jagarlamudi Nethra; B. Srinivasulu; Vadada Vinay Kumar; C. Lakshmana Rao (2024). Microgreens: A Comprehensive Review Emphasizing Urban Agriculture. International Journal of Environment and Climate Change. https://doi.org/10.9734/ijecc/2024/v14i124615 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Daria Barańska, Jacek Panek, Sylwia Różalska, Katarzyna Turnau, Magdalena Frąc

Scientia Horticulturae · 2025 · https://doi.org/10.1016/j.scienta.2025.114303

Abstract

As the global population is projected to surge to 9.78 billion by 2064, urbanization and dietary shifts pose significant challenges to food security. Positioned between sprouts and baby greens, microgreens – especially vegetable microgreens, boast rich bioactive compounds believed to combat chronic diseases. As “superfoods,” they’ve become culinary darlings, embraced by chefs for their flavors and nutritional punch. Their suitability for indoor farming, minimal cultivation resource requirements, and short production cycles make microgreens an ideal addition to functional foods in the era of global urbanization. However, addressing their limited shelf-life and high sensitivity to abiotic stresses requires ongoing research. This review presents the biochemical composition, health-promoting properties, and pre- and post-harvest strategies for enhancing the quality and shelf-life of microgreens, positioning them as an important addition to global diets and the functional food sector. Additionally, it emphasizes the role of the microgreens’ microbiome in boosting resistance to abiotic stresses and extending shelf-life, further enhancing their value and sustainability. Ensuring consistent quality control measures will not only guarantee richness in flavor, texture, and nutritional content but also in shelf-life, resistance, and overall post-harvest quality of microgreens. Understanding the interactions between microgreens and microorganisms holds the potential to develop innovative, environmentally friendly approaches to their cultivation and preservation. Currently, there is a lack of literature on the holobiont approach of microgreens. Therefore, conducting research to increase knowledge about the role of metabolic, genetic, and morphological properties of selected microbe strains in shaping resistance to abiotic stresses and microgreens post-harvest quality is needed.

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Daria Barańska; Jacek Panek; Sylwia Różalska; Katarzyna Turnau; Magdalena Frąc (2025). Microgreens as the future of urban horticulture and superfoods, supported by post-harvest innovations for shelf-life increase: a review. Scientia Horticulturae. https://doi.org/10.1016/j.scienta.2025.114303 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Muhammad Asif Ali, Noohela Khan, Nabeeha Kaleem, Waqas Ahmad, Salem Hussain Alharethi, Bandar Alharbi, Hassan H. Alhassan, Maher M. Al-Enazi, Ahmad Faizal Abdull Razis, Babagana Modu, Daniela Călina, Javad Sharifi‐Rad

Frontiers in Oncology · 2023 · https://doi.org/10.3389/fonc.2023.1168321

Abstract

. However, broccoli sprouts are the chief source of sulforaphane and are 20 to 50 times richer than mature broccoli as they contain 1,153 mg/100 g. SFN is a secondary metabolite that is produced as a result of the hydrolysis of glucoraphanin (a glucosinolate) by the enzyme myrosinase. This review paper aims to summarize and understand the mechanisms behind the anticancer potential of sulforaphane. The data was collected by searching PubMed/MedLine, Scopus, Web of Science, and Google Scholar. This paper concludes that sulforaphane provides cancer protection through the alteration of various epigenetic and non-epigenetic pathways. It is a potent anticancer phytochemical that is safe to consume with minimal side effects. However, there is still a need for further research regarding SFN and the development of a standard dose.

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Muhammad Asif Ali; Noohela Khan; Nabeeha Kaleem; Waqas Ahmad; Salem Hussain Alharethi; Bandar Alharbi; Hassan H. Alhassan; Maher M. Al-Enazi; Ahmad Faizal Abdull Razis; Babagana Modu; Daniela Călina; Javad Sharifi‐Rad (2023). Anticancer properties of sulforaphane: current insights at the molecular level. Frontiers in Oncology. https://doi.org/10.3389/fonc.2023.1168321 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Chiara Amitrano, Sara De Francesco, Marco Durante, Walter Tinganelli, Carmen Arena, Veronica De Micco

Plants · 2024 · https://doi.org/10.3390/plants13243541

Abstract

Numerous challenges are posed by the extra-terrestrial environment for space farming and various technological growth systems are being developed to allow for microgreens’ cultivation in space. Microgreens, with their unique nutrient profiles, may well integrate the diet of crew members, being a natural substitute for chemical food supplements. However, the space radiation environment may alter plant properties, and there is still a knowledge gap concerning the effects of various types of radiation on plants and specifically on the application of efficient and rapid methods for selecting new species for space farming, based on their radio-resistance. Thus, the hypotheses behind this study were to explore the following: (i) the pattern (if any) of radio-sensitivity/resistance; and (ii) if the morphological parameters in relation with pigment content may be a feasible way to perform a screening of radiation responses among species. To perform this, we irradiated dry seeds of basil, rocket, radish, and cress with iron (56Fe; 1550 MeV/(g/cm²)) and carbon (12C; 290 MeV/u, 13 keV/µm) heavy ions at the doses of 0.3, 1, 10, 20, and 25 Gy to investigate the growth responses of microgreens to acute radiation exposure in terms of morphological traits and photosynthetic pigment content. Results indicate that the microgreens’ reaction to ionizing radiation is highly species-specific and that radiation is often sensed by microgreens as a mild stress, stimulating the same morphological and biochemical acclimation pathways usually activated by other mild environmental stresses, alongside the occurrence of eustress phenomena. Over extended periods, this stimulus could foster adaptive changes, enabling plants to thrive in space.

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Chiara Amitrano; Sara De Francesco; Marco Durante; Walter Tinganelli; Carmen Arena; Veronica De Micco (2024). Morphological and Photosynthetic Pigment Screening of Four Microgreens Species Exposed to Heavy Ions. Plants. https://doi.org/10.3390/plants13243541 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Yingjian Lu, Wen Dong, Tianbao Yang, Yaguang Luo, Pei Chen

Molecules · 2021 · https://doi.org/10.3390/molecules26113247

Abstract

Broccoli microgreens have shown potential health benefits due to their high glucosinolate (GL) levels. Previously, we observed that postharvest UVB treatment did not have much effect on increasing GLs in broccoli microgreens. In this study, we investigated the influence of preharvest UVB irradiation on GL levels in broccoli microgreens. UHPLC-ESI/ITMS analysis showed that preharvest UVB treatments with UVB 0.09 and 0.27 Wh/m2 significantly increased the glucoraphanin (GLR), glucoerucin (GLE), and total aliphatic GL levels by 13.7 and 16.9%, respectively, in broccoli microgreens when measured on harvest day. The nutritional qualities of UVB-treated microgreens were stable during 21-day storage, with only small changes in their GL levels. Broccoli microgreens treated before harvest with UVB 0.27 Wh/m2 and 10 mM CaCl2 spray maintained their overall quality, and had the lowest tissue electrolyte leakage and off-odor values during the storage. Furthermore, preharvest UVB 0.27 Wh/m2 treatment significantly increased GL biosynthesis genes when evaluated before harvest, and reduced the expression level of myrosinase, a gene responsible for GL breakdown during postharvest storage. Overall, preharvest UVB treatment, together with calcium chloride spray, can increase and maintain health-beneficial compound levels such as GLs and prolong the postharvest quality of broccoli microgreens.

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Yingjian Lu; Wen Dong; Tianbao Yang; Yaguang Luo; Pei Chen (2021). Preharvest UVB Application Increases Glucosinolate Contents and Enhances Postharvest Quality of Broccoli Microgreens. Molecules. https://doi.org/10.3390/molecules26113247 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Qingming Li, David Llewellyn, Yun Kong, Youbin Zheng

Preprints.org · 2023 · https://doi.org/10.20944/preprints202305.1017.v1

Abstract

Our previous studies showed pure blue (B) spectra from LEDs can promote shade avoidance responses (SARs). However, it is unknown whether SARs vary among different peak wavelengths (λpeak) of B or how they compare with other photomorphologically-important wavebands, such as ultraviolet-A and far-red. To answer these questions, mustard (Brassica juncea, ‘Ruby Streaks’) and arugula (Brassica eruca, ‘Rocket’) seedlings, grown to the cotyledon unfolding stage under the following narrowband spectrum treatments: UVA (λpeak = 385 nm), B1 (λpeak = 404 nm), B2 (λpeak = 440 nm), B3 (λpeak = 455 nm), and FR (λpeak = 730 nm). Both red (R, λpeak = 660 nm) and dark (D) were used as control treatments. The spectrum treatments were provided at 50 μmol m−2 s−1 on a continuous basis. There were no differences among the B treatments except for reduced mustard fresh weight (FWt) in B3. Compared with R, the B treatments promoted hypocotyl elongation, reduced cotyledon size, and increased petiole length in arugula and B1 increased petiole length in mustard. Compared with the B treatments, UVA inhibited hypocotyl and petiole elongation, similar to or greater than R in both species. Compared with the other LED treatments, seedlings grown under FR generally had the lowest hypocotyl and petiole elongation and the smallest cotyledons. Compared to the LED treatments, D substantially promoted hypocotyl elongation and reduced cotyledon size, except compared to FR in arugula. Among the spectrum treatments, the three B treatments had the greatest SAR promotion effects in both species. Despite having the lowest phytochrome activity, FR inhibited SARs normally associated with high FR environments. Legacy parameters used to estimate SAR-promoting effects of spectrum treatments may not be appropriate for characterizing narrowband spectra from LEDs – new approaches must be developed.

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Qingming Li; David Llewellyn; Yun Kong; Youbin Zheng (2023). Narrowband Blue LEDs with Different Peak Wavelengths Similarly Promote Shade Avoidance Responses and Have Greater Promotion Effects Than Ultraviolet A and Far Red in Two Species of Microgreens. Preprints.org. https://doi.org/10.20944/preprints202305.1017.v1 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Luís Puente, Cielo Char, Devansh Patel, Malinda S. Thilakarathna, M. S. Roopesh

Sustainability · 2024 · https://doi.org/10.3390/su16156645

Abstract

This article presents a general overview of scientific publications in the field of microgreens using bibliometric tools. Data were collected from the Web of Science database (from Clarivate Analytics) in the period from 2004 to 2023, covering 20 years of scientific publications. The results are presented in the form of tables, graphs, and charts to analyze the development of microgreens publications. The countries with the greatest influence on the microgreens topic are the USA, Italy, and India, which have the highest number of publications in the analyzed period with 133, 76, and 38 publications, respectively. On the other hand, the authors with the highest number of publications are Raphael, Y. (University Naples Federico II-Italy), De Pascale, S. (University Naples Federico II-Italy), and Luo, Y. (ARS, Food Quality Laboratory, Environmental Microbial &amp; Food Safety Lab, USDA-USA). The journals with the highest productivity in microgreens are HortScience (American Society of Horticultural Science), Horticulturae (MDPI), and Foods (MDPI), with publication numbers of 49, 27, and 23, respectively. Regarding the relationship of the documents in this study with United Nations Sustainable Development Goals (SDGs), the large majority of documents can be linked to SDG 2 (Zero Hunger), followed by SDG 13 (Climate Action) and SDG 3 (Good Health and Well Being). As a final remark, the mapping, trends, and findings in this work can help to establish logical paths for researchers in the field of microgreens.

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Luís Puente; Cielo Char; Devansh Patel; Malinda S. Thilakarathna; M. S. Roopesh (2024). Research Trends and Development Patterns in Microgreens Publications: A Bibliometric Study from 2004 to 2023. Sustainability. https://doi.org/10.3390/su16156645 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Ivana Mezeyová, Alžbeta Hegedűsová, Marcel Golian, Alena Andrejiová, Miroslav Šlosár, Ján Mezey

Agronomy · 2022 · https://doi.org/10.3390/agronomy12051096

Abstract

The study monitored the effect of differentiated mineral nutrition of microgreens species by solution of sodium selenate (2 mg Se/L) on the content of Se, chlorophylls, and other minerals. Chlorophylls were measured spectrophotometrically, Se by electrothermal atomic absorption method (ETAAS) with Zeeman-effect background and elements’ concentration was performed by a dual Inductively coupled plasma atomic emission spectroscopy (ICP-OES) iCAP7600 instrument. The content of selenium in fresh weight moved on average from 0.013 to 12.556 μg/g. Selenisation increased the content of Se in all tested species significantly (p &lt; 0.05) without impacting yield. The content of chlorophyll a moved from 249.9 mg/kg (Mizuna) to 604.4 mg/kg (Arugula) with significant differences between the species, without significance (p ≤ 0.05) between tested variants. The influence of selenisation on other minerals significantly differed (p &lt; 0.05) due to the genetic variability. A significant (p ≤ 0.05) increase in Ca was observed in green basil (10.7%) and cress (20.9%); of Fe in green basil (1.6%) and cress (40.9%); of K in arugula (1.6%), green basil (3.9%) and cress (2.8%); of Zn in arugula (2.6%), green basil (8.6%), cress (2.7%) and radish (5.9%); and of Ba in green basil (5.6%) and cress (23.9%).

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Ivana Mezeyová; Alžbeta Hegedűsová; Marcel Golian; Alena Andrejiová; Miroslav Šlosár; Ján Mezey (2022). Influence of Microgreens Biofortification with Selenium on Their Quantitative and Qualitative Parameters. Agronomy. https://doi.org/10.3390/agronomy12051096 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Rui Wang, Zhenhui Ren, Yamin Li

PubMed · 2025 · https://doi.org/10.17179/excli2025-8239

Abstract

Autism spectrum disorder (ASD) is a complex neurodevelopmental disorder lacking effective treatments. This systematic review and meta-analysis assesses the efficacy and safety of sulforaphane (SFN) for ASD. Eight databases were searched from inception to September 2024, identifying six randomized controlled trials for inclusion. Efficacy outcomes included ASD symptoms measured by the mean difference (MD) or standardized mean difference (SMD), while safety outcomes included adverse events measured by relative risk. Risk of bias was assessed using the Cochrane tool, and evidence certainty was evaluated via the Grade of Recommendations Assessment Development and Evaluation (GRADE). Results showed that SFN significantly improved total symptoms (SMD = -0.27, 95 % confidence interval (CI), -0.42, -0.12), aberrant behavior (SMD = -0.43, 95 % CI, -0.66, -0.19), hyperactivity (SMD = -0.58, 95 % CI, -1.03, -0.13), social interaction (SMD = -0.43, 95 % CI, -0.59, -0.27), social communication (SMD = -0.24, 95 % CI, -0.35, – 0.12), and restricted and repetitive behaviors (RRB) (SMD = -0.16, 95 % CI, -0.31, -0.00). Effects on irritability, anxiety, sensory sensitivity, total social skills, social awareness, social cognition, and social motivation were not statistically significant. Adverse events were similar between intervention and control groups. In conclusion, SFN shows potential in improving ASD symptoms without significant adverse effects. However, results should be interpreted cautiously due to potential influences from assessment tools, outcome assessors, and treatment duration. Further research is needed to confirm the long-term efficacy and safety of SFN for ASD.

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Rui Wang; Zhenhui Ren; Yamin Li (2025). The Effect of Sulforaphane on Autism Spectrum Disorder: Systematic Review and Meta-Analysis. PubMed. https://doi.org/10.17179/excli2025-8239 Licensed under Creative Commons Attribution SA 4.0 (https://creativecommons.org/licenses/by-sa/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Maria Maleva, Galina Borisova, Chrisantus Ahamuefule, Mohamad Darkazanli, Adarsh Kumar

BIO Web of Conferences · 2024 · https://doi.org/10.1051/bioconf/202412102008

Abstract

The use of plant growth-promoting rhizobacteria (PGPR) along with additional biofortification of agricultural plants with scarce essential elements, such as iodine, is a promising area of agricultural biotechnology. The seeds of Pisum sativum L. (var. Madras) pre-inoculated for two hours with PGPR Arthrobacter sp. strain CTF1 (10 8 CFU/mL) were grown for 14 days in a hydroponic culture at foliar spraying with iodine solution (0.01% KI or KIO3) on the 7th day of the vegetation. Growth parameters such as the length of shoot, fresh and dry biomass of seedlings were studied, the germination percentage and vigor index were calculated, and the content of photosynthetic pigments in pea leaves was assessed. The results showed inoculation of pea seeds with PGPR strain CTF1 had a positive effect on the biomass of two-week-old pea microgreens and their vigor index. At the same time, a significant increase in photosynthetic pigments was also observed in the leaves of pea seedlings, especially chlorophyll a (by almost 25%) and carotenoids (by almost 40%). Additionally, application of iodine via foliar spraying, irrespective of its form (KI or KIO3), resulted in nearly a 26-fold surge in amount of microgreens. However, the significant effect of such iodine treatment had a positive effect only on the content of carotenoids.

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Maria Maleva; Galina Borisova; Chrisantus Ahamuefule; Mohamad Darkazanli; Adarsh Kumar (2024). Effect of PGPR Arthrobacter sp. CTF1 and foliar iodine spraying on pea microgreens growth in hydroponic culture. BIO Web of Conferences. https://doi.org/10.1051/bioconf/202412102008 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Sylvain Charlebois

Journal of Agricultural Studies · 2018 · https://doi.org/10.5296/jas.v6i2.12885

Abstract

Microgreens are considered as an emerging superfood, which are young seedlings of vegetables and herbs, produced in seven to fourteen days. Known as “vegetable confetti”, they gained popularity in upscale restaurants. But microgreens’ nutritional value is only starting to be identified through scientific research. Microgreens are nutrient-dense and make a healthy addition to salads, sandwiches, dishes, and other portable food solutions. According to some recent studies, vitamin and mineral levels can exceed full grown vegetables by more than forty times, requiring less water and energy throughout the process. This case study is about a company called Greenbelt Microgreens, based in Hamilton, Canada. Greenbelt Microgreens grows, harvests and distributes certified organic microgreens. The aim of the case study is to better understand the model and how it could be expanded beyond the region by capitalizing on a growing trend of local, organically grown food products. The case presents how microgreens are positioned in the marketplace. It also describes the company itself, its challenges and a discussion on specific, strategic elements to consider.

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Sylvain Charlebois (2018). Can Greenbelt Microgreens Expand its Model? A Discussion on the Future of Microgreens. Journal of Agricultural Studies. https://doi.org/10.5296/jas.v6i2.12885 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Emir Sultan Irmak, Ali Efe Turan, Figen Taşcı Durgut, Funda Eryılmaz Açıkgöz, Fatma Funda Özdüven

International Journal of Agriculture Environment and Food Sciences · 2026 · https://doi.org/10.31015/jaefs.2026.1.11

Abstract

This study aimed to determine the effects of LED lights with different wavelengths on the growth parameters, fresh weight, and total antioxidant content of red cabbage microgreens (Brassica oleracea L. var. capitata subvar. rubra) grown under controlled conditions. The experiment was conducted using a fully automated plant growth chamber, named “Confetti Cabin”, with four light treatments: red LED, blue LED, red+blue LED, and a natural light control group. The study was designed with a randomized plot design and three replications. The highest stem length was observed under red LED light (59.28 mm), the highest fresh weight under red+blue LED (90.70 g), and the highest antioxidant content under blue LED (0.99 EC50 µg/mL). The findings suggest that light spectrum selection can be optimized based on production targets in microgreen cultivation.

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Emir Sultan Irmak; Ali Efe Turan; Figen Taşcı Durgut; Funda Eryılmaz Açıkgöz; Fatma Funda Özdüven (2026). Effects of different LED light wavelengths on the growth and antioxidant activity of red cabbage microgreens (Brassica oleracea L. var. capitata subvar. rubra). International Journal of Agriculture Environment and Food Sciences. https://doi.org/10.31015/jaefs.2026.1.11 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Mónica Flores, Cristian Hernández-Adasme, María José Guevara, V.H. Escalona

Frontiers in Sustainable Food Systems · 2024 · https://doi.org/10.3389/fsufs.2024.1349423

Abstract

Microgreens are vegetable or edible herb shoots harvested in the early stages of development. They have an important number of bioactive compounds and add color, texture, and flavor to dishes and salads. Given their benefits, small size, and high market prices, they can grow in indoor systems, where light is determinant. This study aimed to evaluate the effect of different light intensities on agronomic characteristics, color, chlorophylls and carotenoids content, and antioxidant activity represented by total phenolic content (TPC), eliminate, and antioxidant capacity (AC) in four Brassicaceae species in two colors (green and red). The experiment was conducted in a controlled light-emitting diode (LED) environment growth chamber (day/night temperatures of 25/20 ± 1.2°C, 16 h photoperiod, and 79 ± 2% relative humidity). Three light intensities were used for microgreen growth with the same LED light spectrum: low (120 ± 5.1 μmol m −2 s −1 ), medium (160 ± 3.6 μmol m −2 s −1 ), and high (210 ± 5.9 μmol m −2 s −1 ). Eight g of the seeds of green and red cultivars of cabbage, kale, mizuna, and mustard were sown in a plastic tray (64 cm x 35 cm x 6 cm) with a mixture of peat and perlite (1:2 = v: v). Overall, the high intensity increased dry matter percentage and dry weight, except in green and red kale and green cabbage cultivars. In contrast, low intensity promoted a larger hypocotyl in all species than with high intensity; moreover, it enhanced the cotyledon area in green and red mizuna. Cabbage, kale, and mustard green cultivars were greener under medium intensity, whereas the low intensity enhanced the purple color of mizuna. In addition, chlorophyll a and b increased under low intensity in most species except the red kale and mustard cultivars. The high intensity raises the antioxidant activity, promoting a higher TPC and AC. The findings revealed that the light intensity generated variations in agronomic characteristics, color, chlorophyll content, and antioxidant activity of Brassicaceae microgreens, and the changes were based on the specific species and cultivars.

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Mónica Flores; Cristian Hernández-Adasme; María José Guevara; V.H. Escalona (2024). Effect of different light intensities on agronomic characteristics and antioxidant compounds of Brassicaceae microgreens in a vertical farm system. Frontiers in Sustainable Food Systems. https://doi.org/10.3389/fsufs.2024.1349423 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Saad Mir, Roberts Krumins, Līva Purmale, Varnika Chaudhary, Bhim Bahadur Ghaley

Agronomy · 2024 · https://doi.org/10.3390/agronomy14122895

Abstract

Light spectrum and intensity is one of the key factors in the production of microgreens in controlled-environment agriculture and is directly related to plant growth and biomass accumulation. Hence, the objective of this research study was to investigate the biomass, growth, and resource use efficiencies (RUEs) in 14 different species of microgreen grown in two light recipes with 209.5 (OSRAM LED) and 45 µmol m−2 s−1 (INSTAGREEN LED) with a 16/8 h light/dark photoperiod in a growth chamber. Under both LEDs, fresh biomass accumulation and the SPAD content were highest in sunflower. Nasturtium recorded the maximum hypocotyl length under both LEDs. The leaf area index (LAI) was significantly higher in mungbean under the INSTAGREEN LED compared to other microgreens, while the maximum LAI was measured in lentils under the OSRAM LED. This shows that the two different LEDs had species-specific effects. The RUE of the cheaper INSTAGREEN LED was more efficient in terms of light and energy use efficiency, while OSRAM LED was more efficient in terms of water and surface use efficiencies. Overall, the results showed that different species of microgreens exhibit different responses to fresh biomass accumulation and SPAD contents in the leaves, demonstrating the diversity of their growth responses. Across both LEDs (OSRAM LED and INSTAGREEN LED), the top performing microgreen in terms of biomass accumulation as well as SPAD contents in the leaves was sunflower. Consequently, a high chlorophyll content in sunflower led to a higher biomass production by enhancing photosynthesis.

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Saad Mir; Roberts Krumins; Līva Purmale; Varnika Chaudhary; Bhim Bahadur Ghaley (2024). Effects of Light Intensity and Spectrum Mix on Biomass, Growth and Resource Use Efficiency in Microgreen Species. Agronomy. https://doi.org/10.3390/agronomy14122895 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Syukriyadin Syukriyadin, Ira Devi Sara, S Syahrizal, Mohd Faizal Fauzan, M Fajri

IOP Conference Series Earth and Environmental Science · 2024 · https://doi.org/10.1088/1755-1315/1356/1/012016

Abstract

Abstract The T5 LED lamp is one form of LED light that can be utilized for indoor microgreen growing. The T5 LED lamp has various advantages, such as great energy efficiency and a long lifespan, and they do not emit excessive heat. This research intends to analyze the growth rate of kangkung (Ipomoea reptans poir) utilizing a T5 LED lamp as the light source. The research was carried out through an experimental design. Kangkung microgreen plants were placed under a T5 LED lamp, positioned 50 cm below the lamp’s surface, and connected to the voltage output supply from solar PV. The results showed that the average value of root growth rate was 26% per day, stem length growth rate was 26% per day, leaf width growth rate was 3.74% per day, leaf length growth rate was 23% per day, petiole length growth rate was 29% per day, the stem diameter growth rate is 4% per day, and the wet weight growth rate is 1.42% per day.

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Syukriyadin Syukriyadin; Ira Devi Sara; S Syahrizal; Mohd Faizal Fauzan; M Fajri (2024). Kangkung microgreen growth analysis under T5 LED lighting using solar PV. IOP Conference Series Earth and Environmental Science. https://doi.org/10.1088/1755-1315/1356/1/012016 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Alicia Rihn, Kellie J. Walters, Natalie R. Bumgarner, Pralhad Bajgain

Journal of Agriculture and Food Research · 2024 · https://doi.org/10.1016/j.jafr.2024.101542

Abstract

Microgreens are functional foods whose nutritional content varies depending upon the production methods used when grown. Consumers may perceive different microgreen production practices as carrying varying levels of risk. Here, an online survey of 820 Tennesseans indicated their perceived risk of consumption and environmental impacts for nutritionally enhanced microgreens grown using eight different production methods (e.g., plant breeding, gene editing, lighting, etc.). Probit models were used to analyze data. Results demonstrate that the different production methods were associated with different risk perceptions. Species selection and lighting were perceived as the safest practice for consumption and the environment. Gene editing and genetic modification were perceived as the least safe for consumption and the environment. Production strategies selected as the best option to enhance nutrition improved perceptions of production practice safety. Existing subjective knowledge of the production methods positively influenced consumers’ safety perceptions. Gender, education and urban residency all impacted perceived risk but varied by production method. Given the heightened interest in nutritious food and environmental health, it is important to understand how consumers perceive different production methods to better inform them about their food choices and encourage sustainable consumption behaviors. In turn, results can aid growers, retailers and other stakeholders as they consider alternative production methods and marketing strategies. – Consumers perceived risk for production practices to enhance microgreen nutrient content vary. – Species selection and lighting were perceived as “safest” for consumption and the environment. – Gene editing and modification were perceived as the “least safe”.

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Alicia Rihn; Kellie J. Walters; Natalie R. Bumgarner; Pralhad Bajgain (2024). Consumers’ risk perceptions of production practices to enhance the nutrient content of microgreens. Journal of Agriculture and Food Research. https://doi.org/10.1016/j.jafr.2024.101542 Licensed under Creative Commons Attribution NC ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Eswaranpillai Uma, Priyadharsini Murugesan, Ponmurugan Karuppiah

Plant Science Today · 2023 · https://doi.org/10.14719/pst.2058

Abstract

Many people suffer from a deficiency of essential micronutrients. Sprouts and microgreens can transform the whole idea of vegetables to resolve the need for a diet with fresh, nutrient-rich, and high content of phyto-compounds necessary for a healthy body. The study’s main objective is to evaluate the growth of 6 different seeds, such as four legumes; fenugreek, mung bean, cowpea, horse gram and two grains, wheat, sorghum microgreens. All the seeds were cultivated in soil, water and coco peat, to estimate and compare the nutritional properties of the selected sprouts vs. microgreens. The growth of microgreens in each medium was evaluated, and the proximate and nutritional properties were analysed. In terms of the growth of microgreens, coco peat medium serves the best, as it retains water for a long and it is porous to provide better aeration for the roots and also the day of harvest is shorter. In terms of the nutritional property of microgreens, soil serves the best, as it contains more nutrients than any other medium. The study results showed sprouts are better sources of proteins and carbohydrates than microgreens. However, microgreens were characterized by a high content of carotenoids, chlorophylls and ascorbic acid. It also exhibiting higher anti-diabetic and anticholinergic activity than sprouts. In addition, the microgreens have more micronutrients like zinc, copper, iron, magnesium, potassium etc., than the sprouts. Finally, microgreens were better growing with coco peat and also sources for functional components for dietary supplements and sustainable agriculture.

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Eswaranpillai Uma; Priyadharsini Murugesan; Ponmurugan Karuppiah (2023). Assess the impact of cultivation substrates for growing sprouts and microgreens of selected four legumes and two grains and evaluation of its nutritional properties. Plant Science Today. https://doi.org/10.14719/pst.2058 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Sanja Fabek Uher, Sanja Radman, Nevena Opačić, Mia Dujmović, Božidar Benko, Denis Lagundžija, Valent Mijić, Lucija Prša, Srđan Babac, Jana Šic Žlabur

Plants · 2023 · https://doi.org/10.3390/plants12112098

Abstract

Microgreens are young plants of various vegetables, medicinal and aromatic plants, cereals and edible wild plants that were first associated with nouvelle cuisine as decoration in dishes due to their attractive appearance and strong flavor. Recently, they have become more sought after in the market due to their high nutritional value. This is due to the growing interest of consumers in a healthy lifestyle that includes a varied diet with emphasis on fresh, functional foods. Nowadays, commercial production of microgreens is shifting to modern hydroponic systems due to their numerous advantages, such as accelerated plant growth and biomass production, earlier harvesting, and more production cycles that positively affect yield and chemical composition. Therefore, the aim of this study was to determine the content of specialized metabolites and antioxidant capacity of hydroponically grown alfalfa (Medicago sativa) cv. ‘Kangaroo’, yellow beet (Beta vulgaris var. conditiva) cv. ‘Yellow Lady’, red cabbage (Brassica oleracea L. var. rubra) cv. ‘Red Carpet’, and fennel (Foeniculum vulgare) cv. ‘Aganarpo’ microgreens. The highest content of total phenols (408.03 mg GAE/100 g fw), flavonoids (214.47 mg GAE/100 g fw), non-flavonoids (193.56 mg GAE/100 g fw) and ascorbic acid (74.94 mg/100 g fw) was found in fennel microgreens. The highest content of all analyzed chlorophyll pigments (Chl_a 0.536 mg/g fw, Chl_b 0.248 mg/g fw, and TCh 0.785 mg/g fw) was found in alfalfa microgreens. However, in addition to alfalfa, high levels of chlorophyll a (0.528 mg/g fw), total chlorophyll (0.713 mg/g fw) and the highest level of total carotenoids (0.196 mg/g fw) were also detected in fennel microgreens. The results suggest that microgreens grown on perlite in floating hydroponics have high nutritional potential as a functional food important for human health and therefore could be recommended for daily diet.

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Sanja Fabek Uher; Sanja Radman; Nevena Opačić; Mia Dujmović; Božidar Benko; Denis Lagundžija; Valent Mijić; Lucija Prša; Srđan Babac; Jana Šic Žlabur (2023). Alfalfa, Cabbage, Beet and Fennel Microgreens in Floating Hydroponics—Perspective Nutritious Food?. Plants. https://doi.org/10.3390/plants12112098 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Yumi Shirai, Yūkō Fujita, Ryota Hashimoto, Kazutaka Ohi, Hidenaga Yamamori, Yuka Yasuda, Tamaki Ishima, Hiroyuki Suganuma, Yusuke Ushida, Masatoshi Takeda, Kenji Hashimoto

PLoS ONE · 2015 · https://doi.org/10.1371/journal.pone.0127244

Abstract

Oxidative stress and inflammation play a role in cognitive impairment, which is a core symptom of schizophrenia. Furthermore, a hallmark of the pathophysiology of this disease is the dysfunction of cortical inhibitory γ-aminobutyric acid (GABA) neurons expressing parvalbumin (PV), which is also involved in cognitive impairment. Sulforaphane (SFN), an isothiocyanate derived from broccoli, is a potent activator of the transcription factor Nrf2, which plays a central role in the inducible expressions of many cytoprotective genes in response to oxidative stress. Keap1 is a cytoplasmic protein that is essential for the regulation of Nrf2 activity. Here, we found that pretreatment with SFN attenuated cognitive deficits, the increase in 8-oxo-dG-positive cells, and the decrease in PV-positive cells in the medial prefrontal cortex and hippocampus after repeated administration of phencyclidine (PCP). Furthermore, PCP-induced cognitive deficits were improved by the subsequent subchronic administration of SFN. Interestingly, the dietary intake of glucoraphanin (a glucosinolate precursor of SFN) during the juvenile and adolescence prevented the onset of PCP-induced cognitive deficits as well as the increase in 8-oxo-dG-positive cells and the decrease in PV-positive cells in the brain at adulthood. Moreover, the NRF2 gene and the KEAP1 gene had an epistatic effect on cognitive impairment (e.g., working memory and processing speed) in patients with schizophrenia. These findings suggest that SFN may have prophylactic and therapeutic effects on cognitive impairment in schizophrenia. Therefore, the dietary intake of SFN-rich broccoli sprouts during the juvenile and adolescence may prevent the onset of psychosis at adulthood.

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Yumi Shirai; Yūkō Fujita; Ryota Hashimoto; Kazutaka Ohi; Hidenaga Yamamori; Yuka Yasuda; Tamaki Ishima; Hiroyuki Suganuma; Yusuke Ushida; Masatoshi Takeda; Kenji Hashimoto (2015). Dietary Intake of Sulforaphane-Rich Broccoli Sprout Extracts during Juvenile and Adolescence Can Prevent Phencyclidine-Induced Cognitive Deficits at Adulthood. PLoS ONE. https://doi.org/10.1371/journal.pone.0127244 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Jafar K. Lone, Renu Pandey, C. Gayacharan

Heliyon · 2024 · https://doi.org/10.1016/j.heliyon.2024.e25870

Abstract

Escalating public health concerns necessitate innovative approaches to food sources. Microgreens, nutrient-rich seedlings of vegetables and herbs, have gained recognition as functional foods. This review explores the evolution of microgreens, cultivation methods, biochemical changes during germination, nutritional content, health benefits, and commercial significance. Comprehensive studies have demonstrated that microgreens have an elevated level of various nutrients. Further, in vitro and in vivo research validated their antioxidant, anticancer, antibacterial, anti-inflammatory, anti-obesity, and antidiabetic properties. Microgreens, termed “desert food,” show promise for sustainable food production in climate-vulnerable regions. This paper synthesizes recent research on microgreens, addressing challenges and gaps in understanding their nutritional content and health benefits. It contributes valuable insights for future research, fostering sustainable agriculture and enhancing understanding of microgreens in human health and nutrition.

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Jafar K. Lone; Renu Pandey; C. Gayacharan (2024). Microgreens on the rise: Expanding our horizons from farm to fork. Heliyon. https://doi.org/10.1016/j.heliyon.2024.e25870 Licensed under Creative Commons Attribution NC ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Bhornchai Harakotr, Lalita Charoensup, Panumart Rithichai, Yaowapha Jirakiattikul, Patlada Suthamwong

Resources · 2025 · https://doi.org/10.3390/resources14050071

Abstract

Selecting suitable crop species is crucial for optimizing the productivity and nutritional content of microgreens. In this study, twenty-three diverse microgreen species, grown under controlled conditions, were analyzed for yield, bioactive compounds, and antioxidant activities. The microgreens were cultivated on a peat substrate in a controlled environment, with a growth period of 6 to 20 days from planting to harvest. Conditions were maintained at 25 ± 2 °C, a 16 h photoperiod, CO2 concentration of 1000 ppm, relative humidity of 60 ± 2%, and the LED light was set at 330 μmol/m2/s PPFD. Results from the analysis revealed that the yield, bioactive compounds, and antioxidant potential differed significantly among the twenty-three microgreen species. Unfortunately, the superior microgreens exhibiting greater values for all studied traits could not be identified. However, the principal component analysis (PCA) clustered red radish, rat-tailed radish, and Chinese kale microgreens, which were high in both yield and bioactive compounds. In contrast, red holy basil and lemon basil microgreens had high levels of these compounds but low yields. Additionally, a high level of anti-tyrosinase activity was observed in garland chrysanthemum, Chinese mustard, and Chinese cabbage microgreens. Therefore, these microgreen species can be utilized individually or in varying ratios to produce bioactive compounds in different concentrations that are suitable for various applications. The information presented in this study provides valuable insights for health-conscious consumers and growers for selecting superior species with functional implications.

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Bhornchai Harakotr; Lalita Charoensup; Panumart Rithichai; Yaowapha Jirakiattikul; Patlada Suthamwong (2025). Yield, Bioactive Compounds, and Antioxidant Potential of Twenty-Three Diverse Microgreen Species Grown Under Controlled Conditions. Resources. https://doi.org/10.3390/resources14050071 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Isabelle Crowe, Karen Charlton, Anne‐Therese McMahon, Indiana Rhind, Katherine Kent

Journal of Human Nutrition and Dietetics · 2025 · https://doi.org/10.1111/jhn.70103

Abstract

AIMS: Nutrition and dietetics students are future change agents for sustainable food systems, yet sustainability education remains limited in university curricula. This study evaluated the impact of an experiential learning activity using an indoor aquaponic system on students’ knowledge, attitudes and behavioural intentions towards sustainable diets. METHODS: An embedded mixed methods pre-post survey captured baseline data on students’ sustainable diet knowledge, attitudes and behaviours, alongside diet quality (using the Australian Recommended Food Score). Students then participated in an interactive experiential learning activity involving an indoor aquaponic system. Post-activity surveys assessed changes in knowledge and behaviour intentions, as well as perceptions of the learning experience. Quantitative data were analysed using chi-square tests, regression and McNemar-Bowker tests; qualitative responses were thematically analysed. RESULTS: At baseline, students (n = 58, 87.9% female) reported limited knowledge of local food systems, though most valued sustainable food practices. Students who placed high importance on sustainable diets were significantly more likely to engage in sustainable dietary behaviours. Diet quality was significantly higher among students who grew their own food, bought locally or purchased seasonal produce (all p < 0.05). Participation in the experiential learning activity significantly improved students’ self-reported knowledge of local food systems (p < 0.001) and increased their intentions towards buying locally-grown (57.9% to 86.8%, p < 0.001) and growing food (36.8% to 78.9%, p < 0.001). Thematic analysis highlighted students’ increased awareness of sustainability complexity, their role as nutrition professionals and the importance of curriculum integration. CONCLUSION: Experiential learning with an indoor aquaponic system enhanced students’ knowledge and intentions related to sustainable diets. Students who engaged in sustainable behaviours had significantly higher diet quality, highlighting the potential dual benefit of curriculum integration. Embedding sustainability-focused learning in nutrition curricula may better prepare students to lead change in food systems.

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Isabelle Crowe; Karen Charlton; Anne‐Therese McMahon; Indiana Rhind; Katherine Kent (2025). The Impact of Experiential Learning Using an Indoor Aquaponic System on Nutrition Students’ Sustainable Food Knowledge and Behaviour. Journal of Human Nutrition and Dietetics. https://doi.org/10.1111/jhn.70103 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Onofrio Davide Palmitessa, Massimiliano Renna, Pasquale Crupi, Angelo Lovece, Filomena Corbo, Pietro Santamaria

Foods · 2020 · https://doi.org/10.3390/foods9050677

Abstract

Microgreens are gaining more and more interest, but little information is available on the effects of the chemical composition of the nutrient solution on the microgreen yield. In this study, three Brassica genotypes (B. oleracea var. italica, B. oleracea var. botrytis, and Brassica rapa L. subsp. sylvestris L. Janch. var. esculenta Hort) were fertigated with three modified strength Hoagland nutrient solutions (1/2, 1/4, and 1/8 strength) or with three modified half-strength Hoagland nutrient solutions with three different NH4:NO3 molar ratios (5:95, 15:85, and 25:75). Microgreen yields and content of inorganic ions, dietary fiber, proteins, α-tocopherol, and β-carotene were evaluated. Micro cauliflower showed the highest yield, as well as a higher content of mineral elements and α-tocopherol (10.4 mg 100 g−1 fresh weight (FW)) than other genotypes. The use of nutrient solution at half strength gave both a high yield (0.23 g cm−2) and a desirable seedling height. By changing the NH4:NO3 molar ratio in the nutrient solution, no differences were found on yield and growing parameters, although the highest β-carotene content (6.3 mg 100 g−1 FW) was found by using a NH4:NO3 molar ratio of 25:75. The lowest nitrate content (on average 6.8 g 100 g−1 dry weight) was found in micro broccoli and micro broccoli raab by using a nutrient solution with NH4:NO3 molar ratios of 25:75 and 5:95, respectively. Micro cauliflower fertigated with a NH4:NO3 molar ratio of 25:75 showed the highest dry matter (9.8 g 100 g−1 FW) and protein content (4.2 g 100 g−1 FW).

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Onofrio Davide Palmitessa; Massimiliano Renna; Pasquale Crupi; Angelo Lovece; Filomena Corbo; Pietro Santamaria (2020). Yield and Quality Characteristics of Brassica Microgreens as Affected by the NH4:NO3 Molar Ratio and Strength of the Nutrient Solution. Foods. https://doi.org/10.3390/foods9050677 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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Leandro L. Coutinho, Tharcísio Citrangulo Tortelli, María Cristina Rangel

Frontiers in Oncology · 2023 · https://doi.org/10.3389/fonc.2023.1089115

Abstract

Cancer is a major public health concern worldwide responsible for high morbidity and mortality rates. Alternative therapies have been extensively investigated, and plant-derived compounds have caught the attention of the scientific community due to their chemopreventive and anticancer effects. Sulforaphane (SFN) is one of these naturally occurring agents, and studies have shown that it is able to target a specific cancer cell population displaying stem-like properties, known as cancer stem cells (CSCs). These cells can self-renewal and differentiate to form highly heterogeneous tumor masses. Notably, most of the conventional chemotherapeutic agents cannot target CSCs once they usually exist in a quiescent state and overall, the available cytotoxic drugs focus on highly dividing cells. This is, at least in part, one of the reasons why some oncologic patients relapse after standard therapy. In this review we bring together studies supporting not only the chemopreventive and anticancer properties of SFN, but especially the emerging anti-CSCs effects of this natural product and its potential to be used with conventional antineoplastic drugs in the clinical setting.

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Leandro L. Coutinho; Tharcísio Citrangulo Tortelli; María Cristina Rangel (2023). Sulforaphane: An emergent anti-cancer stem cell agent. Frontiers in Oncology. https://doi.org/10.3389/fonc.2023.1089115 Licensed under Creative Commons Attribution 4.0 (https://creativecommons.org/licenses/by/4.0/). Hosted by EVEC Athens (microgreens.org.gr). No changes were made to this article. Provided as-is, without warranties. EVEC Athens is not affiliated with, and this copy is not endorsed by, the authors or the publisher.

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